Benutzer:Ute.liebelt/Enzymicals
Company Profile[Bearbeiten | Quelltext bearbeiten]
Enzymicals is based in the Hanseatic and University town of Greifswald/Germany. The focus of Enzymicals is the development of resource efficient, sustainable and cost effective solutions for the chemical and pharmaceutical industry. We offer a broad choice of enzymes suitable for research, development and production of fine and special chemicals. Our main task is the development of strategies for the biocatalytic synthesis of customer desired compounds, which includes feasibility studies as well as the processes development orientated on customer demands. We produce high-value chemicals and intermediates up to the kilogram scale under GMP conditions. Enzymicals is integrated into a network which covers the whole value chain from identification and improvement of biocatalysts, process development and optimization up to commercial production, distribution and regulatory issues.
Dedicated to design, develop and implement innovative cost-effective and scalable chemo-biocatalytic routes to fine chemicals, APIs and intermediates, Enzymicals offers recognized expertise in the use of enzymatic processes for complex chemicals synthesis.
History and Collaborations[Bearbeiten | Quelltext bearbeiten]
Enzymicals AG was founded in August 2009 as a spin-off from the University of Greifswald. Already shortly after the founding the Zwingenberg-based biotechnology company BRAIN AG recognized the potential of Enzymicals AG and acquired a minority share as a strategic investment. This strategic alliance combines the complementary modules of both companies to make up the value chain for biocatalytic production of fine and special chemicals. After three years of existence the company got a growth financing from Braun Beteiligungs GmbH, with which, amongst others, the cooperation with Herbrand PharmaChemicals GmbH, belonging to the BBG group, should be strengthened. In cooperation with BRAIN AG and Herbrand PharmaChemicals GmbH fine chemicals, e.g. active pharmaceutical ingredients can be developed within this network starting from the screening of suitable enzymes for production up to the marketable product. Further on Enzymicals AG cooperates with a number of other companies and institutes. One of the most important collaboration is the cooperation with the working group of Prof. Bornscheuer at university in Greifswald.
The customers comes from different fields of the industry with applications e.g. in pharma, flavour and nutrition, polymer industry, diagnostic and also in food.
Products:[Bearbeiten | Quelltext bearbeiten]
Enzymicals focuses on the three segments Enzymes (production of biocatalysts), Chemicals (Synthesis of fine and special chemicals) and Solutions (customer-specific contract research). This focusing is based on a large know-how in biocatalysis and in protein engineering of enzymes for organic synthesis.
Enzymes:[Bearbeiten | Quelltext bearbeiten]
Enzymicals AG has a unique feature in the recombinant pig liver esterases, (R)-selective amine transaminases and Baeyer-Villiger mono- oxygenases which we sell through our catalog business. We offer in-house projects e. g. with newly developed halohydrin dehalogenases and imin reductases and other classes of enzymes. All products in the catalogue are recombinant from Escherichia coli. For the sale of enzymes Enzymicals has established a cooperation with Sigma Aldrich and a registration in the supplier-list of CAS.
- Esterases:
- Various microbial carboxyl esterases
- Isoenzymes of pig liver esterase
- Oxygenases:
- Baeyer Villiger monooxygenases
- Transaminases:
- (R)- and (S)-selective Transaminases
Chemicals:[Bearbeiten | Quelltext bearbeiten]
Enzymicals offers a broad range of chiral building blocks and specialty chemicals with a production capacity from analytical to kilogram scale. The product range includes chiral secondary and tertiary alcohols, carboxylic acids, lactones, esters and amines. We produce these special substances on customer request.
Solutions:[Bearbeiten | Quelltext bearbeiten]
We discover, customise and produce enzymes based on the demands of our partners. Our technology platform covers identification of novel enzymes from strain collections and metagenome libraries, their cloning, the functional expression in a range of microbial hosts and protein engineering tools. Furthermore, we develop synthesis and work-up strategies to apply the biocatalyst in an optimised process.
Customer Development and Manufacturing Service:[Bearbeiten | Quelltext bearbeiten]
Enzymicals service includes modular projects via compilation of segments[Bearbeiten | Quelltext bearbeiten]
- Quantitative analytical set-up
- Spectrophotometry
- Chromatography
- Identification
- Screening of enzyme or strain collection
- Amplification of genes from genomic DNA
- Metagenome screening
- De-novo genome sequencing
- Expression
- General studies for functional expression
- Optimized expression
- Scale up to 100-L
- Purification
- Characterization
- Catalytic properties and kinetic parameter
- Stability, temperature- and pH-profiles
- Structural investigations (PAGE, folding, crystallization)
- Improvement
- Strategy via modeling or structurally inspired
- Rational Design
- Construction and characterization of variants
- Directed Evolution
- Setup of HTS-Assay incl. MTP-expression
- Library construction, expression and screening
- Application
- Screening of customers chemicals for tailor made biotransformation
- Process design and optimization of biotransformation
- Characterization of processes regards economically and sustainable parameters
- Biocatalysis up to 100L scale
- Production of tailor made chemicals
External Links:
- Website Enzymicals AG http://www.enzymicals.com
- Enzymicals-Product portfolio http://www.enzymicals.com/Enzymicals_Enzymes_v011.pdf
- Company-Flyer http://www.enzymicals.com/Enzymicals_Flyer.pdf