Figure: Evolutionary relationship of Conferticium
The evolutionary history was inferred using the Minimum Evolution method [1]. The optimal tree with the sum of branch length = 0.34213088 is shown. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) are shown next to the branches [2]. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the Kimura 2-parameter method [3] and are in the units of the number of base substitutions per site. The ME tree was searched using the Close-Neighbor-Interchange (CNI) algorithm [4] at a search level of 2. The Neighbor-joining algorithm [5] was used to generate the initial tree. The analysis involved 20 nucleotide sequences. All positions with less than 95% site coverage were eliminated. That is, fewer than 5% alignment gaps, missing data, and ambiguous bases were allowed at any position. There were a total of 1179 positions in the final dataset. Evolutionary analyses were conducted in MEGA6 [6]

1. Rzhetsky A. and Nei M. (1992). A simple method for estimating and testing minimum evolution trees. Molecular Biology and Evolution 9:945-967.
2. Felsenstein J. (1985). Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39:783-791.
3. Kimura M. (1980). A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution 16:111-120.
4. Nei M. and Kumar S. (2000). Molecular Evolution and Phylogenetics. Oxford University Press, New York.
5. Saitou N. and Nei M. (1987). The neighbor-joining method: A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4:406-425.
6. Tamura K., Stecher G., Peterson D., Filipski A., and Kumar S. (2013). MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution30: 2725-2729.

List of GenBank-Sequences

• Conferticium ochraceum AF506383
• Conferticium ravum AF506382
• Acanthophysellum lividocoeruleum
• Aleurodiscus cerussatus AF506399
• Xylobolus frustulatus strain AF506491
• Gloeomyces graminicola AF506448
• Stereum rugosum AF506481
• Gloeocystidiellum heimii AF506381
• Stereum hirsutum AF506479
• subtomentosum AF506482
• Stereum reflexulum AF506480
• Gloeocystidiellum triste AF506442
• Megalocystidium luridum AF506422
• Aleurobotrys botryosus AF506398
• Gloeocystidiopsis flammea AF506437
• Megalocystidium leucoxanthum AF506420
• Gloeocystidiellum wakullum AF506443
• Aleurodiscus amorphus AF506397
• Gloeodontia discolor AF506445
• Gloeodontia pyramidata AF506446